Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Enteroviral meningitis reduces CSF concentration of Aβ42, but does not affect markers of parenchymal damage

doi: 10.1007/s10096-019-03569-0

Figure Lengend Snippet: Demographic, laboratory, and CSF characteristics of the studied groups

Article Snippet: Protein S100B was analyzed with Human S100B ELISA kit (Elabscience, Bethesda, MD, USA).

Techniques:

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 1. Expression levels of S100B in normal and tumor tissue. The expression of S100B was assessed from the Oncomine database. (A) TCGA breast dataset; the bars are labeled as follows: 1, breast (normal, n=61); 2, invasive ductal breast carcinoma (n=389); 3, invasive breast carcinoma (n=76); mixed lobular and ductal breast carcinoma (n=7); 5, invasive lobular breast carcinoma (n=36). (B) The Ma et al breast dataset (19); the bars are labeled as follows: 1, breast carcinoma (n=14); 2, ductal breast carcinoma in situ (n=9); 3, invasive ductal breast carcinoma (n=9). Boxplot shows the z-score of S100B expression according to the raw data from Oncomine. Error bars represent standard deviation (SD). The statistically significant differences between each group were examined with one-way ANOVA (***P<0.001). (C) S100B expression in different types of breast cancer examined via the GOBO database. (D) S100B expres- sion was examined in estrogen receptor (ER)-negative (ER-neg) and ER-positive (ER-pos) breast cancer using the GOBO database. The number above each bar indicates the sample size in each group. (E) mRNA expression of S100B in breast cancer cell lines. The error bars represent SD.

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Labeling, In Situ, Standard Deviation

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 2. S100B treatment inhibits cell migration. Transwell migration assay in (A) MDA-MB-231 and (B) Hs578T and (C) MCF-7 cells. Images and quanti- fication results are shown. Images of wound healing assay in (D) MDA-MB-231 and (E) MCF-7 cells. The quantification of wound healing assay in both cells is shown in the lower panel in the images. Error bars represent SD (one-way ANOVA; **p<0.01 and ***p<0.001).

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Migration, Transwell Migration Assay, Wound Healing Assay

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 3. S100B treatment induces mesenchymal-epithelial transition (MET) in MDA-MB-231 cells. (A and B) Western blot analysis was used to examine the protein expression levels of MET signaling pathway-related molecules. Band intensity was normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The relative value of the control (0 nM) was set to 1. Error bars represent SD (t-test; *p<0.05 and **p<0.01).

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Western Blot, Expressing, Control

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 4. Evaluation of the association between S100B expression and survival curve. (A) S100B expression in the low-risk (green) and high-risk (red) group. The box plot is adapted from the SurvExpress database and shows S100B expression in patients. (B) Kaplan-Meier distant recurrence curve was created using the SurvExpress database to analyze the sample from a GEO dataset (GSE9893). The low-risk (n=67) and high-risk (n=67) groups are shown in green and red, respectively. (C) Analyzing the overall survival rate in all breast cancer patients, (D) estrogen receptor (ER)-positive breast cancer patients, and (E) ER-negative breast cancer patients in ‘breast invasive carcinoma TCGA’ from the SurvExpress database. The green and red lines indicate a high and low S100B expression, respectively. Evaluation of the distant metastasis-free survival (DMFS) curve comparing the patients with a high (red) and low (black) S100B expression in (F) all breast cancer patients, (G) ER-positive breast cancer patients, (H) ER-negative breast cancer patients and (I) endocrine therapy-treated breast cancer patients using the KM plotter database. The hazard ratio (HR) and log-rank P-values are shown in each panel.

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 5. Evaluation of whether p53 expression in human breast cancer is associated with S100B expression and patient survival. (A) Expression of S100B in patients with or without p53 mutation. Raw data were adapted from the TCGA breast dataset. Distant metastasis-free survival (DMFS) analysis in breast cancer patients with (B) p53 mutation and (C) p53 wild-type. The hazard ratio (HR) and log-rank P-value are shown in each panel.

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Mutagenesis

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 6. Summary of the S100B-mediated effects in breast cancer. Patients with breast cancer expressing high levels of S100B exhibited a good prognosis and a low metastatic rate. When the breast cancer cells are treated with S100B, the migration ability was inhibited and the epithelial phenotype was induced.

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Migration

Journal: bioRxiv

Article Title: Catch-and-Display Immunoassay as an Accessible Platform for Digital Biomarker Detection

doi: 10.64898/2026.01.27.702166

Figure Lengend Snippet: (A) Representative fields of view for the blank sample (0 µg/mL) and S100B positive (0.2 µg/mL) samples. Pronounced colocalization between immunocomplex fluorescence and nanoparticle hotspots is observed only in the presence of S100B. (B) Digital analysis shows dose-dependent response with saturation at 0.75 µg/mL and linear detection from 0.02–0.75 µg/mL (R² = 0.9889). (C) Analog analysis based on total fluorescence enables linear quantification beyond 0.5 µg/mL, complementing digital readout and expanding the dynamic detection range. Data are presented as mean ± SD (N = 6 spots on a single device). Statistical significance was evaluated using one-way ANOVA with Dunnett’s test (****p < 0.0001, ***p = 0.0007).

Article Snippet: The following reagents and materials were used in this study: anti-S100B (TA700560 and TA600560), anti-GFAP antibody pairs (TA700041 and TA600039), and S100B (TP300277) were all purchased from Origene.

Techniques: Fluorescence

Journal: bioRxiv

Article Title: Catch-and-Display Immunoassay as an Accessible Platform for Digital Biomarker Detection

doi: 10.64898/2026.01.27.702166

Figure Lengend Snippet: (A) Representative fields of view of S100B-negative (no S100B input) and S100B-positive samples (0.1 µg/mL). In the presence of S100B, pronounced colocalization between immunocomplex fluorescence and nanoparticle hotspots is observed, indicating successful formation of target-specific immunocomplexes, whereas negligible colocalization is detected in the absence of S100B. (B) Digital Analysis of the TSA-Modified CAD-IA. Dose response analysis demonstrates sensitive and reproducible detection of S100B with a sensitivity down to 0.0002 µg/mL, at least 100-fold higher than CAD-IA not using TSA. Linear regression of the digital signal output shows a strong correlation between colocalization percentage and S100B concentration across the dynamic range of 0.0002–1 µg/mL (R² = 0.9663). Data are presented as mean ± SD (N = 6 spots on a single device). Statistical significance was evaluated using ordinary one-way ANOVA with Dunnett’s multiple comparisons test (****p < 0.0001, ***p = 0.0002).

Article Snippet: The following reagents and materials were used in this study: anti-S100B (TA700560 and TA600560), anti-GFAP antibody pairs (TA700041 and TA600039), and S100B (TP300277) were all purchased from Origene.

Techniques: Fluorescence, Modification, Concentration Assay

Journal: Nature

Article Title: Innervation of Thermogenic Adipose Tissue via a Calsyntenin-3β/S100b Axis

doi: 10.1038/s41586-019-1156-9

Figure Lengend Snippet: a, Proteomics of WT and Clstn3β KO BAT. b, TUBB3 Immunostaining of sympathetic neurons +/- S100b (n=25 cells). c, d, e, Histology, triglyceride quantitation ( c ), TH staining ( d ) and cold tolerance test ( e ) of Clstn3β KO +/- adiponectin-cre mice receiving AAV-DIO-S100b (n=4 mice). f, TH and TUBB3 immunostaining of BAT from WT, S100b KO and Clstn3β transgenic/S100b KO mice (n=4 mice). g, h, Immunostaining ( g ) and Pearson’s correlation analysis ( h ) of S100b and KDEL in brown adipocytes (n=35 cells). i, Western blot analysis of conditioned media from brown adipocytes. j, Binding assay of S100b and Clstn3β/Clstn3. Scale bar: 100 μm.

Article Snippet: The resin was then incubated with 10 μg/ml recombinant S100b (R&D Systems, 1820-SB-050) in binding buffer at 4°C for 30 min and then washed binding buffer for 3 times, 5 min each.

Techniques: Immunostaining, Quantitation Assay, Staining, Transgenic Assay, Western Blot, Binding Assay

Journal: Nature

Article Title: Innervation of Thermogenic Adipose Tissue via a Calsyntenin-3β/S100b Axis

doi: 10.1038/s41586-019-1156-9

Figure Lengend Snippet: a, b, qPCR analysis of S100b expression in ( a ) various fat depots and in ( b ) inguinal subcutaneous WAT from mice acclimated to RT or 4°C (n=4 mice). c, qPCR analysis of S100b expression in control or Prdm16-transgenic inguinal subcutaneous WAT (n=4 mice). d, qPCR analysis of S100b expression in control or Prdm16-KO inguinal subcutaneous WAT (n=4 mice). e, Prdm16 ChIP-seq showing binding at the S100b locus. f, Indirect calorimetry analysis of Clstn3β KO mice with or without adiponectin-cre receiving AAV-DIO-S100b AAV injection (n=4 mice). g, TH immunostaining of salivary gland from WT and S100b KO mice. h, qPCR analysis of S100b expression in WT and Clstn3β KO BAT from mice housed at RT (n=4 mice). Note this is a different housing condition from . i, Western blot analysis of intracellular level of S100b in Clstn3β KO brown adipocytes expressing S100b lone or co-expressing with Clstn3β. j, Western blot analysis of S100b protein level in HEK293T cells transfected with various constructs as indicated. k, Western blot analysis of S100b and CFD secretion from HEK293T cells co-transfected with or without Clstn3β. All data are mean ± SEM. Statistical significance was calculated by unpaired Student’s two-sided t-test.

Article Snippet: The resin was then incubated with 10 μg/ml recombinant S100b (R&D Systems, 1820-SB-050) in binding buffer at 4°C for 30 min and then washed binding buffer for 3 times, 5 min each.

Techniques: Expressing, Control, Transgenic Assay, ChIP-sequencing, Binding Assay, Injection, Immunostaining, Western Blot, Transfection, Construct